Lyophilized protein vials

Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, His Tag, Sf9 Insect Cells Recombinant Protein

A31141010
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$225.00
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Description

Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, Sf9 Insect Cells Recombinant Protein is a single, glycosylated polypeptide chain containing 164 amino acids (33-187 aa) and has a molecular mass of 19.1 kDa. The M-CSF is fused to a 6 amino acid His tag at C-terminus and is purified by proprietary chromatographic techniques.

Summary

Macrophage colony-stimulating factor (M-CSF) is a cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: UniProtKB - P07141 (CSF1_MOUSE)].

Other Names

Bioactive Murine M-CSF, CSF-1, CSF1 gene product, MCSF

Amino Acid Sequence

ADPKEVSEHC SHMIGNGHLK VLQQLIDSQM ETSCQIAFEF VDQEQLDDPV CYLKKAFFLV
QDIIDETMRF KDNTPNANAT ERLQELSNNL NSCFTKDYEE QNKACVRTFH ETPLQLLEKI
KNFFNETKNL LEKDWNIFTK NCNNSFAKCS SRDVVTKPHH HHHH.

Source

Baculovirus infected Sf9 (Spodoptera frugiperda) insect cells.

Formulation

Sterile Filtered colorless solution. The M-CSF solution (1 mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).

Purity

Greater than 90.0% as determined by SDS-PAGE.

Biological Activity

Determined by cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. ED50 range for this effect is ≤ 4 ng/ml.

Shipping

Shipped on regular ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store at 4°C if entire vial will be used within 2-4 weeks. For longer periods of time, store frozen at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Usage

This product is intended to Laboratory Research Use Only.  This product may not be used a drug, agricultural product, food additives.

Resources

Want to learn more about M-CSF? We have compiled the links below which contain information that you may find interesting.