Lyophilized protein vials

Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein

A3308002
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Description

Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and has a total molecular mass of 37.1 kDa. Human M-CSF is purified by proprietary chromatographic techniques.

Summary

Macrophage colony-stimulating factor (M-CSF) is a cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: UniProtKB - A0A024R0A1 (CSF1_HUMAN)].

Other Names

Bioactive Human M-CSF, CSF-1, CSF1 gene product, MCSF

Amino Acid Sequence

MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS.

Source

E. coli

Formulation

Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized with 10mM sodium Phosphate, pH-8.0 and 50mM NaCl.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Biological Activity

The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells, was found to be 1.15 ng/ml corresponding to a specific activity of 8.7x105 Units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized M-CSF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Shipping

At ambient temperature.  Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized M-CSF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles. 

Usage

This product is intended to Laboratory Research Use Only.  This product may not be used a drug, agricultural product, food additives.

Resources

Want to learn more about M-CSF? We have compiled the links below which contain information that you may find interesting.