Description
ISOkine® M-CSF Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 158 amino acids and an N-terminal 16 amino acid histidine tag for a total length of 174 amino acids and has a predicted molecular mass of 20.52 kDa.
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Summary
Macrophage colony-stimulating factor (M-CSF) is a hematopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. MCSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoietic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody dependent cell-mediated cytotoxicity, enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological activity on murine cells but murine M-CSF is inactive in human cells.
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
2xPBS pH 7.2, sterile filtered through 0.22 µm filter
Purity
> 95% by SDS-PAGE gel analysis.
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human M-CSF is assayed by measuring its dose-dependent effect on the proliferation of M-NFS-60 cells. The ED50 for this effect using M-CSF is typically <4 ng/ml. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Endotoxin level is < 0.01 EU per μg of ISOkine® product by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Synonyms
CSF-1, CSF1 gene product, MCSF.
Documents
Product References
- Tavano, R et al. “Species differences in opsonization and phagocyte recognition of preclinical poly-2-alkyl-2-oxazoline-coated nanoparticles.” Nature communications vol. 16,1 2642. 18 Mar. 2025, doi:10.1038/s41467-025-57648-2
- Glaziou, Quentin et al. “IL-27 neutralization enhances macrophage polarization and improves immune checkpoint efficacy.” bioRxiv (2025), https://doi.org/10.1101/2024.09.13.612803
- d'Almeida, Sènan M et al. “The ecto-ATPDase CD39 is involved in the acquisition of the immunoregulatory phenotype by M-CSF-macrophages and ovarian cancer tumor-associated macrophages: Regulatory role of IL-27.” Oncoimmunology vol. 5,7 e1178025. 28 Apr. 2016, doi:10.1080/2162402X.2016.1178025
- de Jong, Emma et al. “Monocyte-derived macrophages do not explain susceptibility to pulmonary non-tuberculous mycobacterial disease.” Clinical & translational immunology vol. 1,11 e2. 14 Nov. 2012, doi:10.1038/cti.2012.1
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Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.