Description
3-D Life FG-Dextran is a component of the 3-D Life Hydrogel system. It contains maleimide-functionalized dextran (Mal-Dextran) that can be used together with PEG-Link (Cat. No. L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up fast gelling, cell-compatible hydrogels.
When crosslinked with PEG-Link or CD-Link, Dextran-based 3-D Life Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. NOTE: Dextran hydrogels crosslinked with HyLink cannot be dissolved by dextranase.
The included 10x CB Buffer (pH 5.5) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells.
For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
3D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.
Quantity
Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link).
Components
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| Mal-Dextran, Lyophilized |
3 x 170 µl* | 30 mmol/L | MSDS |
| 10x CB Buffer (pH 5.5) | 600 µl | n.a. | MSDS |
| Water | 4 x 1500 µl | n.a. | MSDS |
| *Volume after reconstitution of lyophilizate. |
Resources
- Product Datasheet (PDS): 3-D Life FG-Dextran Component
- Protocol: Preparation of 3-D Life Fast (FG) Gelling Hydrogels
- Protocol: Degradation of dextran-based hydrogels with dextranase
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Application Notes
- Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels
- Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.