Mesenpan: xeno-free, serum-free medium for human mesenchymal stem cells (hMSC) expansion, cell culture media kit (basal medium + growth supplement)

Mesenpan: xeno-free, serum-free medium for human mesenchymal stem cells (hMSC) expansion

P08-50400K
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$286.00
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Introduction

Mesenpan Special Medium is a xeno-free, serum-free medium that was developed especially for proliferation of human mesenchymal stem cells (hMSC) without cell differentiation. It allows a long time culture from hMSC while preserving the differentiation potential in different cell types (multi-lineage potential). To reach a differentiation of the expanded hMSC into different lines, the suitable differentiation factors and protocols should be used.

Mesenpan Special Medium Kit for hMSC without FBS (Cat. No. P08-50400K):

Cell Type: MSCs
Liquid / Powder: liquid
Product Category: Serum-Free Media
Size: 500 ml kit
Sterile: Yes
Manufacturer PAN-Biotech GmbH
Country of Origin Germany

Content

The Mesenpan Kit (Cat. No. P08-50400K) consists of:

  • 500 ml Mesenpan Basal Medium
  • 5 ml Mesenpan Growth Supplement.

Composition

Mesenpan contains salts, amino acids, trace elements, hormones and growth factors in an optimized formulation.

Application

Xeno-free, serum-free cultivation and expansion of human mesenchymal stem cells (hMSC) while maintaining their undifferentiated state and multi-lineage potential.

Please note: For research use only, not for therapeutic or diagnostic use. 

Advantage

Mesenpan is a xeno-free, serum-free complete medium which can be supplemented with 2 % FBS or hPL if necessary. Because of the defined composition of the Basal Medium and the Growth Supplement, stable experimental conditions with a high reproducibility of results are achieved without tedious batch-to-batch testing. The addition of suitable reagents after successful expansion allows a differentiation of the MSC in the desired cell type (e.g., bone, cartilage, muscular tissue).

    Storage conditions & stability

    • Mesenpan Basal Medium (P08-50400B): store in the dark at 2-8° C.
    • Mesenpan Growth Supplement (P08-5040S1): store in the dark at -20° C.

    Mesenpan Basal Medium and Mesenpan Growth Supplement are guaranteed stable for 12 months when stored properly. Mesenpan complete medium (Basal Medium + Growth Supplement) is stable for at least 3 months when stored in the dark at 2-8 °C.

    Documents

    Product References

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    • Nasehi, Ramin et al. “3D geometry orchestrates the transcriptional landscape of metastatic neuroblastoma cells in a multicellular in vitro bone model.” Materials today. Bio vol. 19 100596. 28 Feb. 2023, doi:10.1016/j.mtbio.2023.100596
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    • Köpf, Marius et al. “Bioprinting-Associated Shear Stress and Hydrostatic Pressure Affect the Angiogenic Potential of Human Umbilical Vein Endothelial Cells.” International journal of bioprinting vol. 8,4 606. 18 Aug. 2022, doi:10.18063/ijb.v8i4.606
    • Luengen, Anja E et al. “Bone Marrow Derived Mesenchymal Stromal Cells Promote Vascularization and Ciliation in Airway Mucosa Tri-Culture Models in Vitro.” Frontiers in bioengineering and biotechnology vol. 10 872275. 17 Jun. 2022, doi:10.3389/fbioe.2022.872275
    • Jatho, Aline et al. “The HIFα-Stabilizing Drug Roxadustat Increases the Number of Renal Epo-Producing Sca-1+ Cells.” Cells vol. 11,4 753. 21 Feb. 2022, doi:10.3390/cells11040753
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    • Duarte Campos, Daniela F et al. “Exploring Cancer Cell Behavior In Vitro in Three-Dimensional Multicellular Bioprintable Collagen-Based Hydrogels.” Cancers vol. 11,2 180. 5 Feb. 2019, doi:10.3390/cancers11020180
    • Ventura Ferreira, Mónica S et al. “Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.” Stem cell research & therapy vol. 9,1 28. 5 Feb. 2018, doi:10.1186/s13287-017-0757-1

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