Collection: 3-D Life Ready-To-Design Hydrogel Kits for 3D Cell Culture

The 3-D Life Hydrogels technology by Cellendes is an integrated system for the generation of biomimetic hydrogels used in 3D cell culture. The 3-D Life system consists of two polymers that form a hydrogel by covalent crosslinking at ambient temperature. The chemical reaction is non-toxic to cells and occurs rapidly, preventing cell settlement before the gel is formed.

The 3-D Life Ready-To-Design Hydrogel Kits contain a thiol-reactive polymer, a crosslinker and a 10x concentrated salt and buffer solution. Cell adhesion peptides, Dextranase for gel dissolution and other gel modifying reagents are available as separate products (see products Adhesion Peptides and Accessories).

• PVA Hydrogel Kits: PVA (polyvinyl alcohol) is a biochemically stable polymer providing long lasting hydrogel stability for long-term cell cultures.
• Dextran Hydrogel Kits: Dextran Hydrogel Kits contain thiol-reactive dextran. Dextran hydrogels can be dissolved by 3-D Life Dextranase for a protease-free cell recovery when crosslinked with PEG-Link or CD-Link. Dextran hydrogels crosslinked with HyLink (Dextran-HA Hydrogel Kit) cannot be dissolved by 3-D Life Dextranase.
• Hyaluronic Acid Hydrogel Kits: Hyaluronic Acid Hydrogel Kits contain thiol-modified hyaluronic acid (HA) as a crosslinker.

Hydrogel composition and properties are indicated in their names for easy hydrogel selection:

PVA or Dextran: PVA (polyvinyl alcohol) is a biochemically stable polymer providing long lasting hydrogel stability for long-term cell cultures. Dextran hydrogels can be dissolved by Dextranase (see Accessories) for a protease-free cell recovery.

CD or PEG: Choose between a cell-degradable (CD) crosslinker and a non-degradable (PEG) crosslinker.

FG or SG: Choose between fast gelling (FG) and slow gelling (SG) hydrogels to adapt the set-up of hydrogels to your experimental requirements.

Hyaluronic acid: Select hydrogels crosslinked with hyaluronic acid.

Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH 3-D Life products.